Benzoic acid

Application Name : HPLC Separation of Carboxylic Acids

Benzoicacid Impurity on Zodiac C8 (1)

Column Name : Primesep B2 Columns

Analytes : Benzoic acid, Nicotinic Acid, Acetylbenzoic Acid

Core-shell mixed-mode columns offer unique selectivity which comes from the presence of two mechanisms – reversed-phase and ion-exchange. The alternative selectivity of mixed-mode columns can be used when order of elution or resolution between compounds needs improvement. Even for neutral compounds a different selectivity can be obtained. A method for common components of headache medications (acetaminophen, caffeine, and benzoate) was developed on our Coresep 100 column. Efficiency and peak shape are comparable to leading brand core-shell reversed-phase columns.

Application Name : Selectivity Difference for Polar Compounds on Core-Shell Column in Reversed-Phase and Mixed-Mode

Column Name : Coresep 100 Columns

Analytes : Acetaminophen, Caffeine, Benzoic acid

Application Name : HPLC Separation of Amino Acids, Bases, Acids, and Neutrals on Obelisc R

Column Name : Obelisc R Columns

Analytes : Phenylalanine, Toluene, Benzoic acid, Benzonitrile, Benzylamine, Trypthophan, Phenol, Pyridine, 2,6-Lutidine

Primesep 100 separates a mixture of polar and nonpolar compounds in one analytical run. The amino acid cysteine; amino acid derivatives L-cystine, 2,2-dimethylcystine, and 2-methylcysteine; the polar acid benzoic acid; and the nonpolar neutral toluene are separated by a gradient using a combination of polar and hydrophobic interactions. The separation method uses a mobile phase mixture of water, acetonitrile (MeCN, ACN) and sulfuric acid (H2SO4) with UV detection at 210 nm.

Application Name : Hydrophobic and Hydrophilic Compound Separation

Column Name : Primesep 100 Columns

Analytes : Toluene, Benzoic acid, Cystine, 2-Methylcysteine, L-Cysteine, 2,2-Dimethylcystine, L-Cystine

Analytical chemists face multiple complex separations everyday. Very often, complex mixtures containing various compounds need to be analyzed in a single run. Traditional reversed-phase chromatography has challenges for retention of polar-neutral, polar-acidic and polar-basic compounds in mixtures with hydrophobic compounds. We have developed a universal screening method for analysis of complex mixtures containing polar-neutral, polarbasic, polar-acidic, hydrophobic-neutral and hydrophobic-basic compounds. The method employs Primesep 200 mixed-mode reversed-phase cation-exchange column and a simple mobile phase containing ACN/water/sulfuric acid. The low pH of the mobile phase helps suppress ionization of polar-acidic compounds, making them slightly hydrophobic. Hydrophobic and hydrophilic neutral compounds are retained by RP mechanism, while basic hydrophilic and basic hydrophobic compounds are retained by cation-exchange mechanism. Abbreviations: PN-polar neutral, PB-polar basic, PA-polar acidic, HN-hydrophobic neutral, HB-hydrophobic basic

Application Name :Universal HPLC-UV Method for Complex Mixtures

Column Name : Primesep 200 Columns

Analytes : Homovanillic acid, Amitriptyline, Uracil, Toluene, Benzoic acid, Benzylamine, DOPA (3,4-dihydroxy-L-phenylalanine), Phenol, 2,6-Lutidine, Epinephrine Sulfonate, Hydroxytryptophan, Tryptophan, 2,3-DHBA, Methylparaben, Ethylparaben

Application Name :

Dual-Mode Separation of Diacid Compounds Using Hydrophobic and Ion-Exchange Mechanisms

HPLC Separation of Amino Acids, Bases, Acids, and Neutrals on Zodiac HST TH

HPLC Separation of Organics Acids

Assay Of Guaifenesin USP Method

Comparative HPLC Separation of Hydrophobic and Hydrophilic Compounds on Mixed-Mode Zodiac HST OG vs Single-Mode C8 Column