Benzoic acid

Application Name : HPLC Separation of Carboxylic Acids

Column Name : Primesep B2 Columns

Analytes : Benzoic acid, Nicotinic Acid, Acetylbenzoic Acid

Core-shell mixed-mode columns offer unique selectivity which comes from the presence of two mechanisms – reversed-phase and ion-exchange. The alternative selectivity of mixed-mode columns can be used when order of elution or resolution between compounds needs improvement. Even for neutral compounds a different selectivity can be obtained. A method for common components of headache medications (acetaminophen, caffeine, and benzoate) was developed on our Coresep 100 column. Efficiency and peak shape are comparable to leading brand core-shell reversed-phase columns.

Application Name : Selectivity Difference for Polar Compounds on Core-Shell Column in Reversed-Phase and Mixed-Mode

Column Name : Coresep 100 Columns

Analytes : Acetaminophen, Caffeine, Benzoic acid

Application Name : HPLC Separation of Amino Acids, Bases, Acids, and Neutrals on Obelisc R

Column Name : Obelisc R Columns

Analytes : Phenylalanine, Toluene, Benzoic acid, Benzonitrile, Benzylamine, Trypthophan, Phenol, Pyridine, 2,6-Lutidine

Primesep 100 separates a mixture of polar and nonpolar compounds in one analytical run. The amino acid cysteine; amino acid derivatives L-cystine, 2,2-dimethylcystine, and 2-methylcysteine; the polar acid benzoic acid; and the nonpolar neutral toluene are separated by a gradient using a combination of polar and hydrophobic interactions. The separation method uses a mobile phase mixture of water, acetonitrile (MeCN, ACN) and sulfuric acid (H2SO4) with UV detection at 210 nm.

Application Name : Hydrophobic and Hydrophilic Compound Separation

Column Name : Primesep 100 Columns

Analytes : Toluene, Benzoic acid, Cystine, 2-Methylcysteine, L-Cysteine, 2,2-Dimethylcystine, L-Cystine

Analytical chemists face multiple complex separations everyday. Very often, complex mixtures containing various compounds need to be analyzed in a single run. Traditional reversed-phase chromatography has challenges for retention of polar-neutral, polar-acidic and polar-basic compounds in mixtures with hydrophobic compounds. We have developed a universal screening method for analysis of complex mixtures containing polar-neutral, polarbasic, polar-acidic, hydrophobic-neutral and hydrophobic-basic compounds. The method employs Primesep 200 mixed-mode reversed-phase cation-exchange column and a simple mobile phase containing ACN/water/sulfuric acid. The low pH of the mobile phase helps suppress ionization of polar-acidic compounds, making them slightly hydrophobic. Hydrophobic and hydrophilic neutral compounds are retained by RP mechanism, while basic hydrophilic and basic hydrophobic compounds are retained by cation-exchange mechanism. Abbreviations: PN-polar neutral, PB-polar basic, PA-polar acidic, HN-hydrophobic neutral, HB-hydrophobic basic

Application Name :Universal HPLC-UV Method for Complex Mixtures

Column Name : Primesep 200 Columns

Analytes : Homovanillic acid, Amitriptyline, Uracil, Toluene, Benzoic acid, Benzylamine, DOPA (3,4-dihydroxy-L-phenylalanine), Phenol, 2,6-Lutidine, Epinephrine Sulfonate, Hydroxytryptophan, Tryptophan, 2,3-DHBA, Methylparaben, Ethylparaben