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Separation of Toluene

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Parabens are common preservatives in pharmaceutical and cosmetic industries. They are esters of p-hydroxybenzoic acid. Method for separation of methyl paraben, propyl paraben, benzonitrile and toluene was developed on a Obelisc R column. All four compounds are neutral and are retained by reverse-phase mechanism. In case of reversed-phase stationary phase, no effect of pH is observed. Retention time for all four compounds changes on an Obelisc R column when pH is changed. pH of the mobile phase affects ionization state of stationary phase. Obelisc R column has C12 carbon chain and carboxylic acid with pKa of 4. At lower pH (pH 2, TFA), carboxylic acid of stationary phase is not ionized and thus adds hydrophobicity to stationary phase. Obelisc R column can be used for analysis of basic, acidic and neutral compounds with suitable detection techniques – UV, ELSD, CAD, LC/MS.

Application Name : HPLC Separation of Methyl Paraben, Benzonitrile, Propyl Paraben, and Toluene on Mixed-Mode and Reverse Phase Columns
Column Name : Obelisc R Columns
AnalytesPropyl paraben, Toluene, Methyl Paraben, Benzonitrile

Primesep 100 separates a mixture of polar and nonpolar compounds in one analytical run. The amino acid cysteine; amino acid derivatives L-cystine, 2,2-dimethylcystine, and 2-methylcysteine; the polar acid benzoic acid; and the nonpolar neutral toluene are separated by a gradient using a combination of polar and hydrophobic interactions. The separation method uses a mobile phase mixture of water, acetonitrile (MeCN, ACN) and sulfuric acid (H2SO4) with UV detection at 210 nm.

Application Name : Hydrophobic and Hydrophilic Compound Separation

Column Name : Primesep 100 Columns

Analytes : Toluene, Benzoic acid, Cystine, 2-Methylcysteine, L-Cysteine, 2,2-Dimethylcystine, L-Cystine

Analytical chemists face multiple complex separations everyday. Very often, complex mixtures containing various compounds need to be analyzed in a single run. Traditional reversed-phase chromatography has challenges for retention of polar-neutral, polar-acidic and polar-basic compounds in mixtures with hydrophobic compounds. We have developed a universal screening method for analysis of complex mixtures containing polar-neutral, polarbasic, polar-acidic, hydrophobic-neutral and hydrophobic-basic compounds. The method employs Primesep 200 mixed-mode reversed-phase cation-exchange column and a simple mobile phase containing ACN/water/sulfuric acid. The low pH of the mobile phase helps suppress ionization of polar-acidic compounds, making them slightly hydrophobic. Hydrophobic and hydrophilic neutral compounds are retained by RP mechanism, while basic hydrophilic and basic hydrophobic compounds are retained by cation-exchange mechanism. Abbreviations: PN-polar neutral, PB-polar basic, PA-polar acidic, HN-hydrophobic neutral, HB-hydrophobic basic

Application NameUniversal HPLC-UV Method for Complex Mixtures

Column Name : Primesep 200 Columns

Analytes : Homovanillic acid, Amitriptyline, Uracil, Toluene, Benzoic acid, Benzylamine, DOPA (3,4-dihydroxy-L-phenylalanine), Phenol, 2,6-Lutidine, Epinephrine Sulfonate, Hydroxytryptophan, Tryptophan, 2,3-DHBA, Methylparaben, Ethylparaben

Application NameReference  Reformate  Standard

Column NameGRS – 1 GC Capillary Column

AnalytesBenzene, Toluene, NaphthaleneEthylbenzene, Propylbenzene,Butylbenzene, Propane2-methylpentane, 2,4-dimethylpentane, o-xylene, Isopentane, 1,2,4-trimethylbenzene2,3-dimethylbutane, Methylcyclopentane, m-xylene, p-xylene, isobutane, 2,2-dimethylbutane, 3-methylpentane, 2,2,3-trimethylbutane, Cyclohexane, n-butane, cyclopentane, n-hexane, 2,2-dimethylpentane, 3,3-dimethylpentane, 2-methylhexane, 2,3-dimethylpentane, 1,1-dimethylcyclopentane, 3-methylhexane, cis-1,3-dimethylcyclopentane, trans-1,3-dimethylcyclopentane, 2,5-dimethylhexane and 2,2,3-trimethylpentane, 2,4-dimethylhexane, 2,3-dimethylhexane, 2-methylheptane, 3,4-dimethylhexane, 2,4-dimethylheptane, 2,6-dimethylheptane, 2,5-dimethylheptane, 2,3-dimethylheptane, n-pentane, 3-ethylpentane, trans-1,2-dimethylcyclopentane, n-heptane,  Ethylcyclopentane, 3,3-dimethylhexane, 2-methyl-3-ethylpentane, 4-methylheptane and 3-methyl-3-ethylpentane, trans-1,2-ethylmethylcyclopentane, n-octane, cis-1,2-ethylmethylcyclopentane and 2,3,5-trimethylhexane, 2,2-dimethylheptane, 2-methyl-4-ethylhexane, 3,3-dimethylheptane and 3,5-dimethylheptane, 3,4-dimethylheptane, 4-ethylheptane, n-nonane, 2,2-dimethylhexane, trans-1,3-ethylmethylcyclopentane, 2-methyloctane, 3-ethylheptane, 3-methyloctane, isopropylbenzene, 1-methyl-3-ethylbenzene, 1-methyl-4-ethylbenzene, 1,3,5-trimethylbenzene, 1-methyl-2-ethylbenzene, 1,2,3, trimethylbenzene, Indane, 1,3-diethylbenzene, 4-methyloctane, 3-ethylhexane, 1,4-dimethyl-2-ethylbenzene, 1,3-dimethyl-4-ethylbenzene, 1,2-dimethyl-4-ethylbenzene, 1,2,4,5-tetramethylbenzene, 1,2,3,5-tetramethylbenzene, 2-methylnaphthalene, 1-methylnaphthalene

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