Epinephrine Sulfonate

Epinephrine (also referred to as adrenaline) is a hormone and neurotransmitter. It is a catecholamine, a sympathomimetic monoamine derived from the amino acids phenylalanine and tyrosine. This method can be used to determine and quantitate epinephrine and epinephrine sulfonate in biological fluids (urine, blood, serum) and drug formulations. Obelisc N columns are used to retain and separate epinephrine and epinephrine sulfonate in mixed-mode hydrophilic interaction chromatography. Epinephrine is retained by the combination of cation-exchange and HILIC mechanisms. Epinephrine sulfonate is retained by HILIC mechanism. Buffer concentration and pH, as well as the amount of acetonitrile, can be used to adjust retention of both compounds. Both compounds can be detected by UV, ELSD and LC/MS. Preparative separation is possible with volatile mobile phases (ammonium formate or ammonium acetate.

Application Name : HILIC Separation of Epinephrine and Epinephrine Sulfonate

Column Name : Obelisc N Columns

Analytes : Epinephrine, Epinephrine Sulfonate

Analytical chemists face multiple complex separations everyday. Very often, complex mixtures containing various compounds need to be analyzed in a single run. Traditional reversed-phase chromatography has challenges for retention of polar-neutral, polar-acidic and polar-basic compounds in mixtures with hydrophobic compounds. We have developed a universal screening method for analysis of complex mixtures containing polar-neutral, polarbasic, polar-acidic, hydrophobic-neutral and hydrophobic-basic compounds. The method employs Primesep 200 mixed-mode reversed-phase cation-exchange column and a simple mobile phase containing ACN/water/sulfuric acid. The low pH of the mobile phase helps suppress ionization of polar-acidic compounds, making them slightly hydrophobic. Hydrophobic and hydrophilic neutral compounds are retained by RP mechanism, while basic hydrophilic and basic hydrophobic compounds are retained by cation-exchange mechanism. Abbreviations: PN-polar neutral, PB-polar basic, PA-polar acidic, HN-hydrophobic neutral, HB-hydrophobic basic

Application Name :Universal HPLC-UV Method for Complex Mixtures

Column Name : Primesep 200 Columns

Analytes : Homovanillic acid, Amitriptyline, Uracil, Toluene, Benzoic acid, Benzylamine, DOPA (3,4-dihydroxy-L-phenylalanine), Phenol, 2,6-Lutidine, Epinephrine Sulfonate, Hydroxytryptophan, Tryptophan, 2,3-DHBA, Methylparaben, Ethylparaben