Intact Proteins

By contrast, IEX can be performed with organic solvents in the mobile phase, making it prospectively compatible with all proteins. See Ion Exchange of Proteins with Organic Solvents.

Using an anion- and a cation-exchange column in series yields a mixed-bed arrangement that retains all proteins. The example above is a crude lysate of THP-1 monocyte cell pellet, obtained with a PolyCAT A™ and a PolyWAX LP™ column. Gradients with volatile salts are possible.

Application Name : HPLC Separation Of Mixed-bed IEX Of THP-1 Monocyte cell Lysate

Column Name : PolyCAT A™ Columns

Analytes : Intact Proteins