Citric acid

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Separation of Citric Acid

Column dimention; 300mm x 6.5mm,9um

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    Column Dimention; 200mm x 300mm

    Test Method :

    Eluent: 0.008 N H2SO4
    Flow Rate: 0.6 ml/min
    Detector: UV 210
    Temperature: 35°C

    Column Dimension:300mmx7.8mm

    Test Method :

    • Eluent: 0.008 N H2SO4
    • Flow Rate: 0.6 ml/min
    • Detector: UV 210
    • Temperature: 35°C

    Citric acid, ascorbic acid and EDTA are commonly used in food and pharmaceutical industry as preservatives. These compounds are very polar in nature. They are weak organic acids with limited UV activity. Retention and separation is achieved on HILIC mixed-mode Obelisc N column. All three compounds are retained by combination of strong HILIC and strong anion-exchange mechanisms. Separation can be monitored by ELSD, LC/MS, UV or Corona CAD. In contrast to other HILIC column, Obelisc N has two ionizable groups basic and acidic which provide ion-exchange interaction in addition to hydrophilic interaction. This allows to use less acetonitrile for HILIC separation.

    Application Name : HILIC Separation of Common Preservatives – Citric Acid, Ascorbic Acid and EDTA

    Column Name : Obelisc N Columns

    Analytes : Citric acid, Ascorbic Acid, EDTA (Ethylenediaminetetraacetic Acid)

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