Ascorbic Acid

Column Dimension:300mmx7.8mm
Test Method:
Eluent: 0.008 N H2SO4
Flow Rate: 0.6 ml/min
Detector: UV 210
Temperature: 35°C

Application Name : Separation Of Organic acid by Zodiac Carb 64H

Column Name : Zodiac Carb 64H Column

Analytes : Succinic acid, Formic acid, Oxalic acid, Citric acid, Tartaric acid, Malic acid, Fumaric acid, Ascorbic Acid

Application Name :

• HPLC Analysis of Ascorbic Acid and Sodium Metabisulfite on Zodiac HST- SB Column
• HPLC Isocratic Analysis of Ascorbic Acid and Sodium Metabisulfite on Zodiac HST- SB Column

Ascorbic acid is a naturally occurring vitamin with antioxidative properties. Ascorbic acid and its sodium, potassium and calcium salts are used as preservatives in many products. Acsorbic acid (Vitamin C) is part of a multi-vitamin composition and various supplements. Ascorbic acid was analyzed on the core-shell mixed-mode column with excellent peak shape and retention control. This method can be used for analysis of vitamin C in different compositions and complex matrices. It can be monitored by UV, ELSD or MS. Coresep SB stationary phase is always positively charged and in case of analysis of biofluids, protein components will come before ascorbic acid.

Application Name : HPLC Analysis of Ascorbic Acid on Core-Shell mixed-Mode Column

Column Name : Coresep SB Columns

Analytes : Ascorbic Acid

Citric acid, ascorbic acid and EDTA are commonly used in food and pharmaceutical industry as preservatives. These compounds are very polar in nature. They are weak organic acids with limited UV activity. Retention and separation is achieved on HILIC mixed-mode Obelisc N column. All three compounds are retained by combination of strong HILIC and strong anion-exchange mechanisms. Separation can be monitored by ELSD, LC/MS, UV or Corona CAD. In contrast to other HILIC column, Obelisc N has two ionizable groups basic and acidic which provide ion-exchange interaction in addition to hydrophilic interaction. This allows to use less acetonitrile for HILIC separation.

Application Name : HILIC Separation of Common Preservatives – Citric Acid, Ascorbic Acid and EDTA

Column Name : Obelisc N Columns

Analytes : Citric acid, Ascorbic Acid, EDTA (Ethylenediaminetetraacetic Acid)

Vitamin C (ascorbic acid) and Vitamins Group B are separated on Obelisc N mixed-mode column. Method can be used in quantitation and determination of polar vitamins in various formulations and dietary supplements. HPLC method can be based on UV, Evaporative Light Scattering Detection (ESLD), RI or MS detection. Effect of sample matrix can be eliminated by changing mobile phase conditions. Buffer concentration, buffer pH and amount of ACN will affect every vitamin differently due to difference in polar and ionic properties.

Application Name : HPLC Separation of Vitamin C, Vitamin Group B, and Related Impurities

Column Name : Obelisc N Columns

Analytes : Ascorbic Acid, Nicotinic Acid, Vitamin B6 (Pyridoxine), Vitamin B2 (Riboflavin), 4-Aminopyridine/Fampridine, 3-Aminopyridine, Isonicotinic Acid, Barbituric Acid

Application Name : HPLC Separation and Retention of Vitamins in Mixed-Mode on Zodiac HST B2

Determination of Acetaminophen, Phenylephrine, and Ascorbic Acid on Zodiac HST S1 Column

HPLC Separation of Organic Acids in HILIC and Anion-Exclusion Mode on Zodiac HST S2

HPLC Separation of Vitamin C, Vitamin Group B, and Related Impurities