ZODIAC HST N
Versatility in Polar and Charged Analyte Separation
Types of Interactions of HST N Stationary phase with different analytes

The Zodiac HST N column is a highly advanced mixed-mode chromatographic solution designed to tackle complex separations of polar, very polar, and charged analytes. Its innovative stationary phase incorporates both positively and negatively charged groups, separated by a hydrophilic ligand chain, ensuring efficient interaction with a wide range of analytes.
Why choose Zodiac HST N column ?
The Zodiac HST N chemistry replaces most of the columns available in the HPLC market such as Reversed phase AQ type columns, Normal phase, IC, HILIC, Ion exchange columns etc It’s an ideal column for faster method development with simple mobile phase for laboratories seeking efficient, flexible, and high-performance separation of challenging analytes in pharmaceutical, biochemical, and environmental applications.
- Multiple Separation Mechanisms: Supports Normal Phase, HILIC, ion-exclusion, cation-exchange, anion-exchange, and hydrogen bonding interactions, offering unmatched versatility.
- Enhanced Selectivity: Charged groups on the ligand are spatially optimized to allow efficient interaction with zwitterions and charged analytes without interference.
- Simultaneous Analysis: Achieves simultaneous separation of charged and neutral organic or inorganic compounds, in a single run, simplifying workflows and eliminating the need for multiple techniques.
- Direct Sample Injection: Allows direct injection without extensive cleanup or dilution, unlike ion chromatography.
- Simultaneous Analysis of charged and neutral organic and inorganic compounds which is difficult on Ion- Chromatography technique is achieved within a single HPLC run on Zodiac HST – N columns
- Broad Mobile Phase Compatibility: Works with water-acetonitrile, methanol, and acidic or buffer-modified phases such as TFA, ammonium acetate, or triethylamine acetate, making it LC-MS and ELSD compatible
- Tuneable Performance: Selectivity and retention can be adjusted by modifying pH, organic content, and buffer strength.
Details Will be updated soon…!
- Separation of Sugars on Zodiac HST N
- HPLC Separation of Vitamin C, Vitamin Group B, and Related Impurities
- HPLC Separation of Potassium, Perchlorate, Methanesulfonic, Chloride, Bromide, and Nitrate Ions on Zodiac HST N
- HPLC Method for Analysis of Ammonium Picrate on Zodiac HST N Column
- HPLC Method for Analysis of Allura Red, Sunset Yellow, Tartrazine in Mountain Dew Live Wire at 2 Wavelengths on Zodiac HST N Column
- HPLC Method for Analysis of Allura Red, Sunset Yellow, Tartrazine in Mountain Dew Live Wire at 1 Wavelength on Zodiac HST N Column
- HPLC Method for Analysis of Dyes in Walgreen’s Night Time Cough on Zodiac HST N Column
- HPLC Method for Analysis of Sunset Yellow in Walgreen’s Daytime Non-Drowsy Cold & Flu on Zodiac HST N Column
- HPLC Method for Analysis of Allura Red in Robitussin Cough & Cold on Zodiac HST N Column
- HPLC Method for Analysis of Dyes in Robitussin Cough + Chest on Zodiac HST N Column
- HPLC Method for Analysis of Allura Red in Children’s Mucinex Multi-Syptom Cold
- HPLC Method for Analysis of Dyes in Dimetapp Cough and Cold
- HPLC Method for Analysis of Brilliant Blue and Allura Red in Children’s Mucinex Night Time Multi-Symptom Cold
- HPLC Method for Analysis of Allura Red in Soft Drinks 5 on Zodiac HST N Column
- HPLC Method for Analysis of Allura Red in Soft Drinks 3 on Zodiac HST N Column
- HPLC Method for Analysis of Brilliant Blue in Gatorade Cool Blue on Zodiac HST N Column
- Separation of Trisaccharides on Zodiac HST N Column
- HPLC Analysis of Trifluoroacetic and Acetic Acid on Zodiac HST N Mixed-Mode HPLC Column
- Method for Analysis of Glyphosate in Apple Juice, Cranberry Juice, Mango Juice and Green Tea
- Method for Analysis of Glyphosate in Cranberry Juice on Zodiac HST N Column
- Method for Analysis of Glyphosate in Green Tea on Zodiac HST N Column
- Retention of Taurine on Zodiac HST N Column
- HPLC Analysis of Azide Ion on Zodiac HST N Column
- HPLC Separation of Barium Cation in the Presence of Citric Acid, Sucrose and Tween 80
- HPLC Separation of Maleic Acid and Pentaerythritol on Zodiac HST N Column
- Retention of Azide Ion on Zodiac HST N Column
- HPLC Method for Analysis of Cetylpyridinium Chloride and Triethylene Glycol on Zodiac HST N Column
- HPLC Separation of Cyclodextrin Sulfobutyl Ether and Hydrophobic Compound
- Comparison of Zodiac HST N, SeQuant?® HILIC, and Bare Silica
- Effect of Both pH and Organic Content on a Separation of Sugars, Amino Acids, and Carboxylic Acids
- HPLC Separation of alpha-Aminobutyric, beta-Aminobutyric, and gamma-Aminobutyric acids on Zodiac HST N Column
- HPLC Separation of Glyphosate Reaction Intermediates and Impurities
- HILIC Separation of Common Preservatives – Citric Acid, Ascorbic Acid and EDTA
- HILIC Retention of 1,5-Naphthalenedisulfonic Acid on Zodiac HST N Mixed-Mode HILIC Columns
- Simultaneous Determination of Sodium and Sulfate Ions on Zodiac HST N Mixed-mode HILIC Column
- HILIC Separation of Aromatic Acids on Zodiac HST N Column
- HILIC Separation of Surfactant Triton X-100 on Zodiac HST N Column
- HILIC HPLC Separation of Polyethylene Glycol (PEG) on Zodiac HST N Column
- HILIC Separation of Carboxymethylcysteine and Betaine on Zodiac HST N Column
- HILIC Separation of Epinephrine and Epinephrine Sulfonate on Zodiac HST N Column
- Separation of Serotonin, Dopamine, and Related Compounds on Zodiac HST N Column
- Effect of Buffer on HPLC Separation of Buffers
- HILIC Separation of Carboxylic Acids on Zodiac HST N Column
- HPLC Method for Separation of Vitamins Group B such as Nicotinic Acid, Pyridoxine, Niacinamide, Pantothenic Acid, Riboflavin on Zodiac HST N Column
- Retention of N-Nitrosarcosine on Zodiac HST N Column
- Retention of TFA on Atlantis and Zodiac HST N Column
- HPLC Retention of Sulfuric Acid on Zodiac HST N Column
- HPLC Analysis of TFA on Zodiac HST N Column
- HPLC Separation of Nucleic Bases at pH 4 and 5 on Zodiac HST N Column
- HPLC Separation of Polar and Hydrophobic Drugs on Zodiac HST N Column
- HPLC Method for the Simultaneous Separation of Melamine and Cyanuric Acid on an Zodiac HST N Column
Ordering Information for HST columns :
Column Diameter | 2.1 mm, 3.2 mm, 4.6 mm, 10 mm, 22 mm |
Column Length | 10 mm, 25 mm, 50 mm, 100 mm, 150 mm, 250 mm, Guard |
Particle Size | 2.7 µm, 3 µm, 5 µm, 10 µm |
Pore Size | 90 Å, 100 Å |
Brand | Zodiac HST |
For example for Zodiac HST A column of particle size of 5 micron, 100Å pore-size, with Column dimension of 250mm x 4.6mm, 5um – The code : ZLS.HSTA.46.250.05.10
Support will be updated very soon…!
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