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Synthetic polypeptides : These too can experience deamidation and dehydration, in addition to missing residues and nonenzymatic cleavage. The selectivity of ion-exchange HPLC nicely complements that of reversed-phase (RPC), often resolving variants that coelute in RPC. The example below shows the analysis of pramlintide® in an accelerated heat stress test. Pramlintide is a synthetic analog of the pancreatic peptide amylin, which complements the action of insulin in regulating the metabolism of glucose. Pramlintide’s sequence is given below in the figure.
All asparagine residues are potential deamidation sites. In this severely degraded sample, a deamidation product was observed for each such site except for Asn31.
Column Name : PolySULFOETHYL A™ Columns
Compound Name : Polypeptides