Monoclonal Antibodies

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Separation of Monoclonal Antibodies

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Monoclonal antibodies: The C-terminal residue of the heavy chains is always a Lys- or Arg- residue. Loss of one of these basic residues leads to earlier elution in cation-exchange, while loss of both basic residues leads to an even earlier-eluting peak. The three-peak pattern seen below is characteristic of monoclonal antibodies. The minor peaks are due to deamidation, oxidation, loss of sialic acid residues from carbohydrate side chains, or disulfide bridges with the wrong connections

Application Name : HPLC Application Of Monoclonal antibodies

Column Name : PolyCAT A™ Columns

Analytes : Monoclonal antibodies

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