HPLC Separation of Amino Acids, Bases, Acids, and Neutrals on Zodiac HST TH

Separating basic, acidic, and zwitterionic compounds in a single reverse-phase HPLC run is often challenging, requiring complex gradients and ion-pairing reagents that may hinder MS compatibility. The Zodiac HST TH column, featuring embedded positive and negative ion-pairs, enables precise tuning for the separation of a wide range of compounds with varying ionic properties. Acids, bases, amino acids, and neutral compounds can be separated isocratically using a simple MS-compatible mobile phase of acetonitrile (ACN), water, and ammonium acetate (AmAc) buffer, with detection possible at 250 nm.