HPLC Method for Separation of Vitamins Group B such as Nicotinic Acid, Pyridoxine, Niacinamide, Pantothenic Acid, Riboflavin on Zodiac HST N Column

Separation of B-group vitamins is accomplished using the Zodiac HST N column operating in mixed-mode HILIC. These vitamins vary significantly in polarity and ionic characteristics, making their separation challenging using conventional techniques. Retention and resolution are finely tuned through optimization of acetonitrile content, buffer strength, and buffer pH. A combination of UV and ELSD detection is employed to monitor the separation effectively.

B vitamins are water-soluble compounds essential for cellular metabolism. When formulated together, they are known as vitamin B complex, while individual forms are identified by specific names (e.g., B1, B2, B6, etc.). This method is suitable for the analysis of both individual B vitamins and B complex formulations. Additionally, it enables the isolation of impurities and degradation products using preparative chromatography.