HPLC Method for Analysis of Cetylpyridinium Chloride and Triethylene Glycol on Zodiac HST N Column

Cetylpyridinium chloride is a hydrophobic basic compound, while triethylene glycol is a hydrophilic neutral molecule. Quantitative analysis of both substances poses a challenge due to their differing chemical characteristics. Using the Zodiac HST N column in mixed-mode HILIC/cation-exchange, both compounds can be effectively retained and analyzed.

Cetylpyridinium chloride is retained primarily through cation-exchange interactions, whereas triethylene glycol is retained via the HILIC mechanism. The mixed-mode capability of the Zodiac HST N column allows for flexible retention strategies based on single or multiple interaction modes, making it a powerful tool for analyzing chemically diverse compounds.

Detection can be performed using a combination of UV and ELSD/CAD detectors to monitor both analytes accurately.