HPLC Method for Analysis of Brilliant Blue in Gatorade Cool Blue on Zodiac HST N Column

Brilliant Blue FCF, also known as Blue 1, is a synthetic dye belonging to the triarylmethane class. In its dry form, it appears as a light-blue powder, which becomes a deeper blue when dissolved in water. Upon ingestion, Brilliant Blue is minimally absorbed through the gastrointestinal tract.

Zodiac HST N is a highly polar HPLC column featuring embedded acidic and basic ionizable groups, enabling effective retention of Brilliant Blue. This method is compatible with UV detection and serves as a versatile solution for the analysis of similar polar dye compounds.