HPLC Method for Analysis of Allura Red, Sunset Yellow, Tartrazine in Mountain Dew Live Wire at 2 Wavelengths on Zodiac HST N Column

Sunset Yellow FCF, also known as Orange Yellow S, C.I. 15985, or Yellow 6, is a petroleum-derived azo dye with a bright yellow appearance when dissolved in water and a reddish-orange hue in powder form. This dye is subject to bans or restrictions in countries such as Norway, Finland, and Sweden.

The Zodiac HST N column, designed with highly polar characteristics and featuring embedded acidic and basic ionizable groups, effectively retains Sunset Yellow. The method is compatible with both LC/MS and UV detection, making it suitable for the analysis of Sunset Yellow and structurally similar compounds.