HPLC Method for Analysis of Allura Red, Sunset Yellow, Tartrazine in Mountain Dew Live Wire at 1 Wavelength on Zodiac HST N Column

Sunset Yellow FCF, also known as Orange Yellow S, C.I. 15985, or Yellow 6, is a synthetic azo dye derived from petroleum. In its powder form, it appears reddish-orange, while in aqueous solution, it turns bright yellow. This dye is banned or restricted in several countries, including Norway, Finland, and Sweden, due to regulatory concerns.

The Zodiac HST N column, featuring highly polar characteristics with embedded acidic and basic ionizable groups, provides effective retention of Sunset Yellow. The method is fully compatible with LC/MS and UV detection, offering a robust analytical approach for Sunset Yellow and other structurally related compounds.