HPLC Method for Analysis of Allura Red in Robitussin Cough & Cold on Zodiac HST N Column

Sunset Yellow FCF, also known as Orange Yellow S, C.I. 15985, or Yellow 6, is a synthetic orange azo dye derived from petroleum. In its solid form, it appears reddish-orange, while in aqueous solution, it becomes bright yellow. This dye is banned or subject to restrictions in countries such as Norway, Finland, and Sweden due to health concerns.

The Zodiac HST N column, engineered with highly polar characteristics and embedded acidic and basic ionizable functionalities, is capable of effectively retaining Sunset Yellow. The method is compatible with both LC/MS and UV detection, providing a versatile solution for the analysis of Sunset Yellow and structurally related polar dyes.