HPLC Method for Analysis of Allura Red in Children’s Mucinex Multi-Syptom Cold

Sunset Yellow FCF, also known as Orange Yellow S, C.I. 15985, or Yellow 6, is an orange azo dye derived from petroleum. In its powdered form, it appears reddish-orange, while in solution, it turns bright yellow. The dye is subject to bans or restrictions in several countries, including Norway, Finland, and Sweden, due to safety concerns.

Zodiac HST N, a highly polar HPLC column with embedded acidic and basic ionizable groups, effectively retains and separates Sunset Yellow. The method is suitable for both LC/MS and UV detection and provides a versatile platform for the analysis of structurally similar polar compounds.