HILIC Retention of 1,5-Naphthalenedisulfonic Acid on Zodiac HST N Mixed-Mode HILIC Columns

Naphthalenedisulfonic acid is effectively retained on the Zodiac HST N mixed-mode HILIC/anion-exchange column through an anion-exchange mechanism. The retention time is primarily influenced by the concentration of counter-ions in the mobile phase. This method delivers excellent peak shape and tunable retention, making it a robust solution for quantifying various sulfonic acids across different sample matrices.

Quantification can be performed using UV, ELSD, or LC/MS detection techniques, depending on sensitivity and sample requirements.