Comparative HPLC Separation of Hydrophobic and Hydrophilic Compounds on Mixed-Mode Zodiac HST OG vs Single-Mode C8 Column

This application highlights the advantage of gradient elution on a mixed-mode Zodiac HST OG column compared to a traditional single-mode C8 column. While the C8 column successfully separated only the polar compounds (#3 and #6), the non-polar compounds co-eluted with minimal resolution. Achieving comparable separation on the C8 column would require the addition of ion-pairing reagents to the mobile phase. In contrast, the Zodiac HST OG mixed-mode column effectively separates both polar and non-polar compounds without the need for such additives, demonstrating its superior versatility and efficiency in complex mixture analysis.