Acetaminophen

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Separation of Acetaminophen

Mobile Phase: A: H2O + 0.1% HCOOH, B: CH3CN + 0.1% HCOOH,
Gradient: 25% – 80% B in 20 mins
Flow: 1.0 ml/min
Detection: UV @ 254 nm

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Column : Zodiac C18
Size : 250mm x 4.6mm,10µm
Mobile Phase : A: H2O + 0.1% HCOOH, B: CH3CN + 0.1% HCOOH
Gradient: 15% – 70% B in 10 mins
Flow : 1.0 ml/min
Detection : UV @ 254 nm

Core-shell mixed-mode columns offer unique selectivity which comes from the presence of two mechanisms – reversed-phase and ion-exchange. The alternative selectivity of mixed-mode columns can be used when order of elution or resolution between compounds needs improvement. Even for neutral compounds a different selectivity can be obtained. A method for common components of headache medications (acetaminophen, caffeine, and benzoate) was developed on our Coresep 100 column. Efficiency and peak shape are comparable to leading brand core-shell reversed-phase columns.

Application Name : Selectivity Difference for Polar Compounds on Core-Shell Column in Reversed-Phase and Mixed-Mode

Column Name : Coresep 100 Columns

Analytes : Acetaminophen, Caffeine, Benzoic acid

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