HPLC Separation of Potassium, Perchlorate, Methanesulfonic, Chloride, Bromide, and Nitrate Ions on Zodiac HST N

Ion chromatography is commonly used to analyze hydrophilic organic and inorganic ions, but similar separation can be achieved using the Zodiac HST N column with its HILIC/mixed-mode functionality. The column surface contains polar groups, one basic and one acidic, connected by a hydrophilic linker under low organic conditions. The Zodiac HST N column supports both cation-exchange and anion-exchange mechanisms, enabling simultaneous separation of positively and negatively charged molecules in a single run. For example, five anions (chloride, bromide, methanesulfonate, nitrate, perchlorate) and one cation (sodium) were separated in a single analysis. This method is compatible with ELSD, CAD, and LC/MS, making it suitable for analyzing various hydrophilic and hydrophobic ions in one HPLC run.