Zodiac HST HB
Zodiac HST HB Column: Advanced Hydrogen Bonding Chromatography
The Zodiac HST HB Column introduces cutting-edge hydrogen-bonding (H-bonding) chromatography, setting a new standard in separation technology. This innovative column is designed to separate molecules based on their capacity to form hydrogen bonds, leveraging a unique stationary phase for unparalleled selectivity and efficiency.
Why Choose Zodiac HST HB Column?
- Unique H-bonding-based separation mechanism for precise analysis of polar and structurally similar molecules.
- Simplifies method development with predictable retention patterns and broad compound compatibility.
- A revolutionary tool for research, method development, and industrial applications, providing unmatched speed, selectivity, and efficiency.
Transform your chromatography workflow with the Zodiac HST HB Column—the next frontier in hydrogen-bonding chromatography.
- Hydrogen Bonding Specificity:
The Zodiac HST HB Column uses a stationary phase engineered to separate compounds primarily through hydrogen bonding interactions. Molecules with polar X-H bonds (e.g., alcohols, amines, acids, phenols, and amides) exhibit higher retention, while hydrocarbons demonstrate minimal retention, ensuring clear separation. - Non-Aqueous Mobile Phase Compatibility:
- Operates with acetonitrile (MeCN) and methanol (MeOH) as the mobile phase, optimizing hydrogen bond interactions.
- MeOH, as a strong solvent, interacts significantly with the stationary phase, modulating analyte retention.
- Wide Application Range:
Suitable for analyzing a broad spectrum of compounds, including isomers, polar molecules, related impurities, oxidation/reduction products, and drug metabolites. - Unique Selectivity:
- Selectivity based on molecular geometry, functional groups, and hydrogen bond interaction points.
- Efficient separation of structurally similar compounds, such as isomers and homologs.
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- Speed:
- The MeCN/MeOH mobile phase combination has 2–3 times lower viscosity than water-based systems.
- Enables high-speed separations with smaller particles, achieving UPLC-like performance on standard HPLC instruments.
- Shortens analysis time up to 5x and further increases speed when using UPLC equipment.
- Solubility:
- MeOH-MeCN mixtures dissolve a wide range of polar and non-polar molecules, from sugars and salts to lipids and surfactants.
- Enhanced Preparative Chromatography:
- Low boiling points of MeCN and MeOH facilitate easy solvent evaporation.
- Mobile phase is MS-friendly, supporting MS-driven sample collection and isocratic methods to minimize solvent consumption.
- Improved Selectivity:
- Ideal for complex separations of compounds with similar properties.
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Method Development :
- Polar Surface Area (PSA):
PSA, of which represents the number of interaction points” available for hydrogen bonding of polar atom surfaces, is a useful indicator for predicting retention patterns of the analytes. While PSA highlights potential H-bonding interactions, proximity and accessibility of these interaction points play a critical role in retention time. - Intra- vs. Inter-Molecular Hydrogen Bonding:
Molecules with intra-molecular hydrogen bonding exhibit reduced retention due to limited inter-molecular interactions with the stationary phase. - pKa Considerations:
Despite its non-aqueous operation, ionic interactions between the stationary phase and analytes contribute to the retention profile.
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