Zodiac HST HB
Advanced Hydrogen Bonding Chromatography

Hydrogen bonding interactions between analytes and the column stationary phase in the presence of acetonitrile as the mobile phase.

Hydrogen bonding interactions between analytes and the column stationary phase in the presence of methanol as the mobile phase.
The Zodiac HST HB Column introduces cutting-edge hydrogen-bonding (H-bonding) chromatography, setting a new standard in separation technology. This innovative column is designed to separate molecules based on their capacity to form hydrogen bonds, leveraging a unique stationary phase for unparalleled selectivity and efficiency.
Why Choose Zodiac HST HB Column?
- Unique H-bonding-based separation mechanism for precise analysis of polar and structurally similar molecules.
- Simplifies method development with predictable retention patterns and broad compound compatibility.
- A revolutionary tool for research, method development, and industrial applications, providing unmatched speed, selectivity, and efficiency.
Transform your chromatography workflow with the Zodiac HST HB Column—the next frontier in hydrogen-bonding chromatography.
- Hydrogen Bonding Specificity:
The Zodiac HST HB Column uses a stationary phase engineered to separate compounds primarily through hydrogen bonding interactions. Molecules with polar X-H bonds (e.g., alcohols, amines, acids, phenols, and amides) exhibit higher retention, while hydrocarbons demonstrate minimal retention, ensuring clear separation. - Non-Aqueous Mobile Phase Compatibility:
- Operates with acetonitrile (MeCN) and methanol (MeOH) as the mobile phase, optimizing hydrogen bond interactions.
- MeOH, as a strong solvent, interacts significantly with the stationary phase, modulating analyte retention.
- Wide Application Range:
Suitable for analyzing a broad spectrum of compounds, including isomers, polar molecules, related impurities, oxidation/reduction products, and drug metabolites. - Unique Selectivity:
- Selectivity based on molecular geometry, functional groups, and hydrogen bond interaction points.
- Efficient separation of structurally similar compounds, such as isomers and homologs.
Advantages :
- Speed:
- The MeCN/MeOH mobile phase combination has 2–3 times lower viscosity than water-based systems.
- Enables high-speed separations with smaller particles, achieving UPLC-like performance on standard HPLC instruments.
- Shortens analysis time up to 5x and further increases speed when using UPLC equipment.
- Solubility:
- MeOH-MeCN mixtures dissolve a wide range of polar and non-polar molecules, from sugars and salts to lipids and surfactants.
- Enhanced Preparative Chromatography:
- Low boiling points of MeCN and MeOH facilitate easy solvent evaporation.
- Mobile phase is MS-friendly, supporting MS-driven sample collection and isocratic methods to minimize solvent consumption.
- Improved Selectivity:
- Ideal for complex separations of compounds with similar properties.
Details Will be updated soon…!
- HPLC Determination of Succinic Anhydride and Succinic Acid on Zodiac HST HB Column
- HPLC separation of Dihydroxybenzoic acid in Hydrogen-Bonding mode on Zodiac HST HB
- HPLC Determination of Piracetam on Zodiac HST HB Column
- HPLC Determination of Imidazolidinyl urea on Zodiac HST HB Column
- HPLC Determination of Allantoin on Zodiac HST HB Column
- HPLC Separation of Acedoben and Inosine on Zodiac HST HB Column
- HPLC Determination of Dicyandiamide on Zodiac HST HB Column
- HPLC Separation of Pyrazine and Aminopyrazine on Zodiac HST HB Column
- HPLC Separation of Mixture of DMF and DMSO on Zodiac HST HB Column
- HPLC Analysis of Ethephon in Hydrogen-Bonding Mode on Zodiac HST HB Column
- Separation of Benzoic and Acetylbenzoic acid in Hydrogen-Bonding Mode on Zodiac HST HB Column
- Paraquat and Diquat Separation in Non-aqueous Mobile Phase on Zodiac HST HB Column
- HPLC Separation of Aminopyridines in Non-Aqueous Mobile Phase on Zodiac HST HB Column
- HPLC Dye Separation on Zodiac HST HB Column
- Retention of Taurine on Zodiac HST HB Column
- Analysis of Compounds of TCA Cycle on Zodiac HST HB Column
- HPLC Separation of Nucleosides and Deoxynucleosides on Zodiac HST HB Column
- HPLC Separation of Inosine and Deoxyinosine on Zodiac HST HB Column
- HPLC Separation of the Nonionic Surfactant Triton-X-100 on Zodiac HST HB Column
- HPLC Separation of Aminopyridines Isomers in Hydrogen-Bonding mode on a Zodiac HST HB HPLC Column
- HPLC Separation of Adenosine and Adenine Using the Hydrogen Bonding Method
- HPLC Separation of Cytidine and Cytosine Using the Hydrogen Bonding Method
- HPLC Separation of Homovanillic Acid, Dopamine, and DOPAC using Hydrogen Bonding
- HPLC Separation of Phthalic Acids using Hydrogen Bonding on Zodiac HST HB Column
- HPLC Separation of Pyrilamine, Trimipramine, Pindolol Using Hydrogen Bonding Mode
- HPLC Separation of Pseudoephedrine, Norephedrine, Phenylephrine, and Norphenylephrine Using Hydrogen Bonding Mode
- HPLC Separation of Caffeine, 3- Methylxanthine, 1- Methylxanthine, Xanthine on Zodiac HST HB Column
- HPLC Separation of Thymidine, Uridine, Adenosine, Guanosine, and Cytidine Using the Hydrogen Bonding Method
Ordering Information for HST columns :
Column Diameter | 2.1 mm, 3.2 mm, 4.6 mm, 10 mm, 22 mm |
Column Length | 10 mm, 25 mm, 50 mm, 100 mm, 150 mm, 250 mm, Guard |
Particle Size | 2.7 µm, 3 µm, 5 µm, 10 µm |
Pore Size | 90 Å, 100 Å |
Brand | Zodiac HST |
For example for Zodiac HST A column of particle size of 5 micron, 100Å pore-size, with Column dimension of 250mm x 4.6mm, 5um – The code : ZLS.HSTA.46.250.05.10
Support will be updated very soon…!
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