Zodiac HST B Column
Best for Acidic-Hydrophobic and Neutral analytes
The Zodiac HST B column is a high-performance, strong basic mixed-mode analytical column designed to separate a broad range of analytes. Featuring basic ion-pairing groups embedded on long ligand chains, this column excels in providing enhanced retention and selectivity, especially for hydrophobic and acidic components in complex mixtures. With a pKa value of 10, it is well-suited for diverse chromatographic applications.
Why Choose Zodiac HST B Column?
The Zodiac HST B column is ideal for complex separations, offering high resolution, reproducibility, and versatility for applications involving acidic and hydrophobic compounds. Its unique selectivity and compatibility with a variety of mobile phases make it a top choice for high-performance chromatographic workflows.
- Mixed-Mode Separation: Combines reverse-phase and anion-exchange mechanisms to effectively separate acidic analytes, offering enhanced selectivity.
- Eliminates Silanol Interactions: Strong bases, like quaternary amines, often face poor performance due to strong silanol interactions. The Zodiac HST B column, with its positively charged surface, eliminates these interactions, ensuring efficient separation and symmetrical peak shapes.
- Unique Selectivity for Basic Compounds: While basic compounds are retained through reverse-phase interactions, the presence of positively charged groups on both the analyte and stationary phases enables unique selectivity via ion-exclusion phenomena.
- Optimal pH Range: Operates within a pH range of 1.0 to 4.5, using acids like trifluoroacetic, phosphoric, perchloric, or formic acids to control the mobile phase.
- Broad Mobile Phase Compatibility: Offers flexibility with a wide range of organic modifiers (0-100%) to adjust retention and selectivity.
- Mobile Phase Compatibility: Fully compatible with LC-MS, preparative chromatography, and low UV detection applications.
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- Analysis of Phosphoserine on Zodiac HST B
- Analysis of Testosterone on Zodiac HST B
- Analysis of Sertraline on Zodiac HST B
- Analysis of Cariprazine on Zodiac HST B
- Analysis of Phthalic Acid on Zodiac HST B
- Analysis of Methyl cholate, Cholic acid, Deoxycholic acid, Chenodeoxycholic acid on Zodiac HST B
- ZODIAC HST Method for Separating Corticosteroids such as Prednisone, Prednisolone and Methylprednisolone on Zodiac HST B Column
- Separation of Vitamin E and Vitamin E Acetate on Zodiac HST B Column
- Dual-Mode Separation of Diacid Compounds Using Hydrophobic and Ion-Exchange Mechanisms
- Dual-Mode HPLC Analysis of Dihydroxybenzoic Acid Isomers
- HPLC Analysis of β‑Hydroxy-β-methylbutyric Acid Using Zodiac HST B Column
Ordering Information for HST columns :
| Column Diameter | 2.1 mm, 3.2 mm, 4.6 mm, 10 mm, 22 mm |
| Column Length | 10 mm, 25 mm, 50 mm, 100 mm, 150 mm, 250 mm, Guard |
| Particle Size | 2.7 µm, 3 µm, 5 µm, 10 µm |
| Pore Size | 90 Å, 100 Å |
| Brand | Zodiac HST |
For example for Zodiac HST A column of particle size of 5 micron, 100Å pore-size, with Column dimension of 250mm x 4.6mm, 5um – The code : ZLS.HSTA.46.250.05.10
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