Zodiac HST B Column

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Zodiac HST B Column

Best for Acidic-Hydrophobic and Neutral analytes
ion exchange columns

The Zodiac HST B column is a high-performance, strong basic mixed-mode analytical column designed to separate a broad range of analytes. Featuring basic ion-pairing groups embedded on long ligand chains, this column excels in providing enhanced retention and selectivity, especially for hydrophobic and acidic components in complex mixtures. With a pKa value of 10, it is well-suited for diverse chromatographic applications.

Why Choose Zodiac HST B Column?

The Zodiac HST B column is ideal for complex separations, offering high resolution, reproducibility, and versatility for applications involving acidic and hydrophobic compounds. Its unique selectivity and compatibility with a variety of mobile phases make it a top choice for high-performance chromatographic workflows.

  1. Mixed-Mode Separation: Combines reverse-phase and anion-exchange mechanisms to effectively separate acidic analytes, offering enhanced selectivity.
  2. Eliminates Silanol Interactions: Strong bases, like quaternary amines, often face poor performance due to strong silanol interactions. The Zodiac HST B column, with its positively charged surface, eliminates these interactions, ensuring efficient separation and symmetrical peak shapes.
  3. Unique Selectivity for Basic Compounds: While basic compounds are retained through reverse-phase interactions, the presence of positively charged groups on both the analyte and stationary phases enables unique selectivity via ion-exclusion phenomena.
  4. Optimal pH Range: Operates within a pH range of 1.0 to 4.5, using acids like trifluoroacetic, phosphoric, perchloric, or formic acids to control the mobile phase.
  5. Broad Mobile Phase Compatibility: Offers flexibility with a wide range of organic modifiers (0-100%) to adjust retention and selectivity.
  6. Mobile Phase Compatibility: Fully compatible with LC-MS, preparative chromatography, and low UV detection applications.

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Ordering Information for HST  columns :

Column Diameter 2.1 mm, 3.2 mm, 4.6 mm, 10 mm, 22 mm
Column Length10 mm, 25 mm, 50 mm, 100 mm, 150 mm, 250 mm, Guard
Particle Size2.7 µm, 3 µm, 5 µm, 10 µm
Pore Size90 Å, 100 Å
Brand Zodiac HST

For example for  Zodiac HST A column of particle size of 5 micron, 100Å pore-size, with Column dimension of 250mm x 4.6mm, 5um – The code :  ZLS.HSTA.46.250.05.10

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