Study of Mobile Phase Composition Impact on Retention of Three Compounds with the Zodiac HST TH Column

This application demonstrates the impact of mobile phase composition on the retention of acidic, basic, and neutral compounds. The retention of basic and acidic compounds is primarily influenced by the pH and concentration of the buffer, while the retention of neutral compounds depends on the percentage of acetonitrile in the mobile phase.

The buffer pH affects not only the ionization state of ionizable analytes, but also the ionization state of the trimodal stationary phase in the Zodiac HST TH column. This interplay allows for fine-tuned control of compound retention across a wide range of chemical classes. The method is compatible with multiple detection techniques including UV, ELSD, LC/MS, and Corona detection.