Sodium

Sulfate and phosphate are separated on Primesep B2 and Primesep D column by anion-exchange mechanism. Because both sulfate and phosphate ions are not UV-active, ELSD is used to monitor separation of both anions. Method can be used to quantitate phosphate and sulfate in various pharmaceutical and chemical formulation, products and solution, drinking and ground water. Retention time is adjusted by increase or decrease of buffer concentrations. Two anions can be retained and separated on a very short column due to strong ion-exchange interaction. Method shows good reproducibility and versatility.

Application Name : HPLC Separation of Sulfate and Phosphate Ions on Mixed-Mode HPLC

Column Name : Primesep B2 Columns

Analytes : Sodium, Phosphat, Sulfate

The ionic forms of Chlorate, Perchlorate, and Phosphonates are useful in many industries including medicine, paper and use in explosives. Due to their lack of UV activity, an ELSD was used to detect both the anions and cations of all three sodium salts. The ions were retained on both Primesep D and Obelisc R columns. Primesep D is a reverse phase column with embedded basic ion-pairing groups. Obelisc R is also a reverse phase column, but can be additionally tuned due to embedded ionic groups and a hydrophobic chain.

Application Name : Separation of Chlorate, Perchlorate, and Phosphonate Ions

Column Name : Obelisc R Columns

Analytes : Sodium, Phosphonate, Chlorate, Perchlorate

Polyethylenimine is an amino-based hydrophilic polymer. Polyethyleneimine are used in the cell culture of weakly anchoring cells to increase attachment. Due to the fact that it is a complex mixture of polymers with different molecular weight it is very hard to obtain a single sharp peak for this compound. Polyethylenimine was analyzed on a Promix MP column. The method can be used for quantitation of polyethyleneimines in plasma, blood, saliva and other biological fluids. ELSD and LC/MS methods can be employed for detection.

Application Name : HPLC Analysis of Polyethylenimine in Plasma

Column Name : Promix MP Columns

Analytes : Sodium, Polyethylenimine