Separation of Sulfonamides and Phenylhydrazine Derivatives Using Zodiac HST OG Mixed-Mode Column

This application demonstrates the efficient separation of a series of sulfonamide compounds and a phenylhydrazine derivative using the Zodiac HST OG mixed-mode stationary phase. The analysis was performed using an isocratic elution with a mobile phase of acetonitrile and water (50:50) and 0.1% trifluoroacetic acid (TFA) as the buffer. The method provides baseline resolution for all five analytes within a 6-minute run time, showcasing the column’s high selectivity and efficiency for polar aromatic compounds. Detection was performed using UV at 210 nm, suitable for sulfonamide functional groups. This method is ideal for quality control or impurity profiling in pharmaceutical or environmental samples.