Separation of Quaternary Amines by Reverse-Phase Mechanism

The Zodiac HST B column enables efficient separation of quaternary amines like t-butylamine with symmetrical peak shapes by leveraging a combination of reversed-phase and ion-exclusion mechanisms. Its embedded basic functional group effectively shields silanol activity, reducing peak tailing. This method produces excellent peak shape and is compatible with mass spectrometry, utilizing a mobile phase of water, acetonitrile (MeCN, ACN), and trifluoracetic acid (TFA), with detection via evaporative light scattering (ELSD)..