Separation of Polar Drugs on Zodiac HST TH Column

Common hydrophobic and hydrophilic basic drugs are effectively separated using mixed-mode chromatography on the Zodiac HST TH column, offering enhanced selectivity and resolution compared to traditional reversed-phase columns. Compounds are retained through a combination of reversed-phase and cation-exchange mechanisms.

Retention times can be precisely adjusted by modifying the acetonitrile content, buffer concentration, and buffer pH. The method supports a variety of detection techniques depending on the buffer system, including UV, Evaporative Light-Scattering Detector (ELSD), Charged Aerosol Detection (CAD), and LC/MS. This approach provides a robust and versatile solution for the analysis of basic drugs and pharmaceutical formulations.