Separation of Pharmaceuticals Using Mixed-Mode Chromatography on Zodiac HST OG

This application demonstrates the efficient separation of pharmaceutical compounds — specifically Albuterol and Amoxicillin — using the Zodiac HST OG, which combines reversed-phase and ion-exchange mechanisms.

The mobile phase includes 30% acetonitrile (MeCN) with 0.15% trifluoroacetic acid (TFA), and detection is performed at UV 210 nm. These conditions allow for selective retention of both hydrophilic and ionizable drugs.

The Zodiac HST OG column’s dual retention capability enables separation of structurally and chemically distinct pharmaceutical compounds within a short run time, ideal for quality control, formulation analysis, and method development in drug research.