Separation of Nine Nucleotides by Mixed-Mode Chromatography Using the Zodiac HST-SB Column

Nucleotides are essential biological molecules that function as the building blocks of nucleic acids. Each nucleotide consists of a five-carbon sugar, a nitrogenous base, and one or more phosphate groups. Due to their highly polar nature, nucleotides are typically not retained on standard reversed-phase columns without the use of ion-pairing reagents. The Zodiac HST SB column overcomes this limitation by incorporating strong embedded basic ion-pairing groups, allowing effective retention and separation of all nine nucleotides using mixed-mode interactions.