Separation of Maleic and Succinic Acid on Zodiac HST B2 Column

Description

Organic and inorganic acids can be effectively retained and separated using the Zodiac HST B2 mixed-mode column, which operates through a combination of weak reversed-phase and weak to moderate anion-exchange mechanisms. The retention times of these acids are influenced by the acetonitrile content, buffer concentration, and buffer pH.

Detection can be performed using low-wavelength UV, ELSD, or LC/MS, depending on the analytes and application needs. The presence of buffer ions is essential to enable the ion-exchange mechanism and enhance retention.

This method serves as a general approach for the analysis of both hydrophilic and hydrophobic acidic compounds, including carboxylic acids and inorganic acids, without the need for ion-pairing reagents. It offers a versatile and efficient solution for challenging separations involving polar and charged analytes.

Condition

1. Succinic Acid
2. Maleic Acid