Separation of Guanylurea and Metformin on Zodiac HST OG Column

HPLC Method for Analyzing Guanylurea and Metformin

Guanylurea, the primary metabolite of metformin, forms through its biotransformation in the liver. While guanylurea has minimal pharmacological activity, understanding this metabolite is crucial for insights into metformin’s pharmacokinetics, safety, efficacy, and potential environmental impact.

Guanylurea and metformin can be effectively retained, separated, and analyzed using the Zodiac HST OG mixed-mode stationary phase column. This method employs a simple mobile phase of water, acetonitrile (MeCN), and sulfuric acid buffer, with UV detection at 200 nm.