Description
HPLC Method for Aminopterin, Methotrexate, and Folic Acid
Aminopterin, methotrexate, and folic acid are chemically related compounds essential in biochemistry and medicine. Methotrexate and aminopterin act as antifolates, inhibiting DHFR and disrupting DNA synthesis, while folic acid (vitamin B9) supports cell growth and DNA repair.
These compounds can be analyzed using a Zodiac HST OG column (4.6 x 150 mm, 5 µm, 100 Å) with a mobile phase of water, acetonitrile (MeCN), and ammonium formate buffer. Detection options include UV at 290 nm, ELSD, CAD, or ESI-MS. LOD varies based on the laboratory setup.
Condition
Column Name : Zodiac HST OG
Compound Name : Methotrexate, Aminopterin, Folic Acid
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