Separation of Antibiotics Using Mixed-Mode Chromatography on Zodiac HST TH Column

A complex mixture of sulfonamide, macrolide, tetracycline, and fluoroquinolone antibiotics was successfully separated in a single run using mixed-mode chromatography on the Zodiac HST TH column under LC/MS-compatible conditions. The separation was achieved through a combination of reversed-phase and ion-exchange interactions, allowing for efficient resolution of compounds with diverse chemical properties. This versatile method is suitable for analyzing multiple classes of antibiotics and their related impurities across various sample matrices, including blood, urine, soil, and wastewater.