Separation of Acetaminophen and 4-Aminophenol on Zodiac HST C

Acetaminophen (paracetamol) and 4-aminophenol are both aminophenol derivatives. Acetaminophen is a widely used analgesic and antipyretic, while 4-aminophenol serves as an intermediate in paracetamol synthesis and is also used in photographic development.

These structurally related compounds were effectively retained and separated using the Zodiac HST C mixed-mode HPLC column, which combines reversed-phase retention with complex-forming functional groups. This dual-mode interaction allows for the resolution of closely related analytes that might otherwise coelute on traditional columns.