Separation of a Mixture of Tryptophan and its Catabolites on Zodiac HST OG Column

Tryptophan and Its Catabolites Analysis Using Zodiac HST OG Column

Tryptophan and its catabolites play crucial roles in protein synthesis, neurotransmission, and immune regulation. Key metabolites include:

• Tryptophan: Essential amino acid, precursor to serotonin and melatonin.

• Serotonin: Neurotransmitter regulating mood, appetite, and sleep.

• Kynurenine Pathway Metabolites:

  • Kynurenine: Intermediate in tryptophan metabolism.

  • Kynurenic Acid: NMDA receptor antagonist with neuroprotective properties.

  • Xanthurenic Acid: Studied for its role in metabolic and neurological disorders.

  • 3-Hydroxykynurenine: Can induce oxidative stress.

  • Quinolinic Acid: NMDA receptor agonist with neuroactive properties.

• Indoleamine 2,3-Dioxygenase (IDO) Pathway: Regulates immune response and cell proliferation.

Tryptophan and its catabolites can be retained, separated, and analyzed using a Zodiac HST OG mixed-mode column with a gradient mobile phase of water, acetonitrile (MeCN), and sulfuric acid buffer, with UV detection at 220 nm.