Separation of a Mixture of Tryptophan and its Catabolites on Zodiac HST OG Column

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Description

Tryptophan and Its Catabolites Analysis Using Zodiac HST OG Column

Tryptophan and its catabolites play crucial roles in protein synthesis, neurotransmission, and immune regulation. Key metabolites include:

  • Tryptophan: Essential amino acid, precursor to serotonin and melatonin.

  • Serotonin: Neurotransmitter regulating mood, appetite, and sleep.

  • Kynurenine Pathway Metabolites:

    • Kynurenine: Intermediate in tryptophan metabolism.

    • Kynurenic Acid: NMDA receptor antagonist with neuroprotective properties.

    • Xanthurenic Acid: Studied for its role in metabolic and neurological disorders.

    • 3-Hydroxykynurenine: Can induce oxidative stress.

    • Quinolinic Acid: NMDA receptor agonist with neuroactive properties.

  • Indoleamine 2,3-Dioxygenase (IDO) Pathway: Regulates immune response and cell proliferation.

Tryptophan and its catabolites can be retained, separated, and analyzed using a Zodiac HST OG mixed-mode column with a gradient mobile phase of water, acetonitrile (MeCN), and sulfuric acid buffer, with UV detection at 220 nm.

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