Separation of 11 Amino Acids Using Zodiac HST OG Column with ELSD Detection

This application demonstrates the simultaneous separation of 11 amino acids using the Zodiac HST OG mixed-mode HPLC column. The mobile phase includes 30% acetonitrile (MeCN) and uses a trifluoroacetic acid (TFA) gradient from 0.05% to 0.3% over 25 minutes, enabling efficient retention and resolution of structurally similar amino acids.

The separation is monitored using Evaporative Light Scattering Detection (ELSD), suitable for non-UV-absorbing compounds such as amino acids. This method exploits both ion-exchange and reversed-phase mechanisms of the Zodiac HST OG column.