Description
Taurine (2-aminoethanesulfonic acid) is a highly polar, zwitterionic compound widely used as an additive in nutritional formulations. Its strong polarity and zwitterionic nature make it unsuitable for analysis using conventional reversed-phase (RP) chromatography. Additionally, taurine lacks chromophores and is not detectable by UV absorbance.
An HPLC method for taurine analysis was developed using the Zodiac HST HB column, which employs hydrogen-bonding as the primary separation mechanism. This method enables effective retention and resolution of taurine. With appropriate modifications, it can be applied to complex sample matrices and is compatible with ELSD or LC/MS detection systems.
Condition
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