Description
Sialic acid, a polar, acidic neuraminic acid derivative, is analyzed using the Zodiac HST RP column. Retention is achieved through weak reversed-phase and anion-exchange mechanisms without requiring ion-pairing reagents. Retention time is adjusted by mobile phase pH and buffer concentration. This method is compatible with ELSD and LC/MS and can analyze sialic acid in biological fluids like blood, serum, and urine.
Condition
Column Name : Zodiac HST RP
Compound Name : Sialic acid
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