Retention of Naproxen on Zodiac HST OG Column

Naproxen, a commonly used nonsteroidal anti-inflammatory drug (NSAID), was successfully retained and separated using the Zodiac HST OG column (4.6 × 150 mm, 5 μm). The separation was achieved under reversed-phase conditions with a mobile phase composed of acetonitrile and water (40:60, v/v) containing 0.1% sulfuric acid as a buffer. The acidic modifier enhanced peak shape and retention of the analyte. A flow rate of 1.0 mL/min and UV detection at 270 nm ensured high sensitivity and reproducibility. This method is suitable for routine analysis of Naproxen in pharmaceutical formulations.