Retention and Separation of Antiviral Drugs Using Zodiac HST OG Column

This application demonstrates the effective separation of three antiviral medications—Acyclovir, Valacyclovir, and Oseltamivir—using the Zodiac HST OG mixed-mode stationary phase column. The analysis utilizes a gradient of sulfuric acid buffer (0.05% to 0.8%) in a MeCN/H₂O (30/70%) mobile phase. The use of a gradually increasing buffer concentration allows fine-tuned control over compound retention. A 3.2 × 100 mm, 5 µm column was used with a flow rate of 0.5 mL/min and UV detection at 255 nm. This method highlights the versatility of the Zodiac HST OG column in resolving structurally and functionally distinct antiviral compounds under mixed-mode chromatography conditions.