Resolution between Domperidone and Droperidol

Column : Zodiac C18

Column Size : 100mm*4.6mm, 3µm

Mobile Phase :
A: Dissolve 5.0g of Ammonium Acetate in 1000ml of HPLC Grade Water
B: Methanol HPLC Grade

Gradient : Time (min) Mobile Phase-A(%V/V Mobile Phase- B(%V/V) 0-10 70 30 10-15 0 100 15-15.01 70 30 15.01-20 70 30

Flow Rate : 1.5ml/min

Column Temperature : 25°C

Wavelength : 280nm

Diluent/Sample Solvent : N,N Dimethylformamide Domperidone Reference Solution (a) : (0.10 and 0.15 mg/ml) Weight accurately about 10mg of Domperidone CRS and 15mg of Droperidol CRS into a 100ml Volumetric flask dissolve and dilute to volume with Diluent and mix Inject 10µl of Domperidone Reference Solution (a) in duplicate and calculate Resolution between Domperidone and Droperidol.