Resolution between Domperidone and Droperidol

Preparation of Mobile Phase :

1.) Mobile Phase : A
     Dissolve 5.0g of Ammonium Acetate in 1000ml of HPLC Grade Water

2.) Mobile Phase : B
      Methanol HPLC Grade

3.) Gradient Program :

4.) Diluent : N,N Dimethylformamide
Domperidone Reference Solution (a) : (0.10 and 0.15 mg/ml) Weight accurately about 10mg of Domperidone  CRS and 15mg of  Droperidol CRS into a 100ml Volumetric flask dissolve and dilute to volume with Diluent and mix Inject 10µl of Domperidone Reference Solution (a) in duplicate and calculate Resolution between Domperidone and Droperidol.

Acceptance Criteria:

Resolution between Domperidone and Dropridol should not be less than 2.0

Column Name : Zodiac C18 Column

Compound Name : Droperidol, Domperidone