Purification Of Variant Glycopeptides

Selectivity in HILIC is the opposite of that of RPC. It is very sensitive to addition or deletion of a Ser- or carbohydrate residue, less sensitive to addition or deletion of a Leu- or Phe- residue. Thus, the two modes are complementary and are sometimes used in sequence to purify complex mixtures. An example is the assessment of the degree of glycation of γ-interferon. The tryptic fragments are resolved on a RPC column. The two glycopeptide peaks are collected and rerun via HILIC on aPolyHYDROXYETHYL A™ column. This resolves each peak into a library of glycopeptide peaks, each differing from its neighbor by one carbohydrate residue.

Column Name : PolyHYDROXYETHYL A™ Columns

Compound Name : Glycopeptides