Protein Variant Analysis

Positional variants: Some proteins are derivatized with polyethylene glycol (PEGylation) to retard their catabolism, prolonging their longevity in circulation. PEG is usually attached to the -SH group in cysteine or an amino group in lysine or at the N-terminus. However, a protein may have more than one -SH or amino group, and a number of products can result that are derivatized to the same extent but at different positions. The example below involves a neurotrophic factor. The main product has a PEG chain on the N-terminus, while the nearby minor variants have PEG chains attached to different Lys- residues. These variants differ dramatically in biological activity.

Application Name : HPLC Application of Positional variants Protein

Column Name : PolyCAT A™ Columns

Analytes : Protein Variant Analysis