Neutral, Base, and Acid on Zodiac HST B Column

The Zodiac HST B column enables the simultaneous separation of acidic, basic, and neutral compounds in a single injection. Compounds such as maleic acid, benzonitrile, and benzylamine are baseline resolved through a synergistic combination of reversed-phase, ion-exchange, and ion-exclusion interactions. The method delivers excellent peak shape using a mass spectrometry-compatible mobile phase consisting of water, acetonitrile (MeCN, ACN), and trifluoroacetic acid (TFA), with detection performed at 210 nm via UV.