Impact of Buffer Strength on Ivermectin Retention Using Zodiac HST OG Column

This study demonstrates the effect of buffer strength on the retention behavior of Ivermectin using the Zodiac HST OG mixed-mode column. Due to its weakly basic nature and moderate hydrophobicity, Ivermectin’s interaction with the stationary phase is influenced by both ionic and hydrophobic mechanisms. By adjusting the buffer concentration in the mobile phase, significant changes in retention time are observed in Doxycycline but unaffecting the Ivermectin, highlighting the role of ionic strength in modulating selectivity and resolution. This method provides a robust approach for optimizing separation conditions for complex, amphiphilic drug compounds like Ivermectin.