Mixed-Mode Retention and Separation of Ammonia, Primary and Secondary Amines on Zodiac HST A Column

Description

Ammonia, primary, and secondary amines are essential building blocks in organic synthesis and are widely used in agriculture, pharmaceuticals, and chemical manufacturing. These basic, hydrophilic compounds often present analytical challenges, including poor peak shape, limited retention on C18 columns, and lack of UV chromophores. The Zodiac HST A mixed-mode column provides effective retention and separation without the need for derivatization or ion-pairing reagents. Using a mobile phase with trifluoroacetic acid, compounds such as ammonia, methylamine, morpholine, ethylamine, diethylamine, and butylamine can be efficiently analyzed and monitored by ELSD, ensuring reliable peak shape and resolution.

Condition