LCMS Method for Analysis of Glucosamine-1-Phosphate and Glucosamine-6-Phosphate on Zodiac HST S1 Column

Glucosamine-1-phosphate and Glucosamine-6-phosphate are two phosphorylated derivatives of glucosamine, an amino sugar integral to the structure of glycosaminoglycans such as chondroitin sulfate and hyaluronic acid.

• Glucosamine-1-phosphate is phosphorylated at the first carbon and plays a role in metabolic pathways leading to the synthesis of UDP-glucose, a precursor in glycogen and glycosaminoglycan biosynthesis. It also serves as an intermediate in amino sugar metabolism.

• Glucosamine-6-phosphate, phosphorylated at the sixth carbon, is a key intermediate in the hexosamine biosynthetic pathway. It contributes to the formation of UDP-N-acetylglucosamine (UDP-GlcNAc), essential for glycoprotein and glycosaminoglycan production, and is implicated in metabolic regulation and insulin signaling.

Both phosphorylated forms are essential to carbohydrate metabolism and cellular function.

Separation and analysis of Glucosamine-1-phosphate and Glucosamine-6-phosphate can be effectively performed using the Zodiac HST S1 mixed-mode stationary phase column. A gradient HPLC method utilizing a straightforward mobile phase of water, acetonitrile (MeCN), and formic acid is employed, with detection carried out via LC-MS.