Ion-Exclusion HPLC Separation of Different Acids Using Zodiac HST OG Column

Organic acids were separated by ion-exclusion chromatography using the Zodiac HST OG column (250 × 4.6 mm). The mobile phase consisted of 25% acetonitrile and 75% water with 0.03% sulfuric acid. The method was run at a flow rate of 0.3 mL/min and monitored by UV detection at 210 nm. This setup enables effective separation of polar acidic compounds and is compatible with LC/MS.