Ibuprofen

Mobile Phase: A: H2O + 0.1% HCOOH, B: CH3CN + 0.1% HCOOH,
Gradient: 25% – 80% B in 20 mins
Flow: 1.0 ml/min
Detection: UV @ 254 nm

Application Name : HPLC Separation of Analgesics

Column Name : Zodiac Phenyl

Analytes : Ibuprofen, Acetylsalicylic acid (Aspirin), Carbamazepine, Naproxen, Diclofenac, Salicylic acid, Tolmetin, Acetaminophen, Acetanilide, Phenacetin

Gemfibrozil is used to lower cholesterol and triglyceride levels in the blood and Ibuprofen is a common over-the-counter painkiller. Fast separations of these two compounds are obtained on short 50 mm columns. Both Primesep B2 and Primesep D separate by a combination of anion exchange and reversed phase. Retention times on Primesep B2 can be adjusted by changing the percentage of acetonitrile. On Primesep D retention times can be changed by adjusting the percentage of acetonitrile and acetic acid in the mobile phase. The HPLC separations use a mobile phase of water, acetonitrile (MeCN, ACN), ammonium formate and acetic acid (HOAc) with UV detection at 270 nm.

Application Name : HPLC Analysis of Gemfibrozil and Ibuprofen Using Mixed-Mode Chromatography

Column Name : Primesep B2 Columns

Analytes : Ibuprofen, Gemfibrozil